S t r u c t u r e   &   O r g a n i z a t i o n :     L e c t u r e  # 5 Vocabulary | Study Questions
Introduction to Cells
Objectives:
  1. Understand the basic concepts of cell theory.
  2. Be able to explain the major differences between prokaryotic and eukaryotic cells.
  1. Basic concepts of cell theory
    Cells: we begin as a single cell and develop into an individual organism of trillions of cells: the basic structural unit of life.
    Most cells are invisible to the naked eye. (Cell theory applies to our current earth environment, not the reducing, primitive earth environment: spontaneous generation.)
    1. All organisms are composed of one or more cells.
    2. The cell is the basic structural and functional unit of life.
    3. All cells arise from pre-existing cells.
      (Shown by Pasteur in the mid 1850's.)
  2. Basic cell types
    Two common types of cells/groups of organisms: prokaryotic and eukaryotic.
    1. Prokarytic cells ("before the nucleus" - primitive cell type)
      figure 5.1: a prokaryotic cell Prokaryotes are a magnitude smaller than eukaryotes. There are two main groups of prokaryotes: bacteria and cyanobacteria. Both have cell membranes, cell walls, and carry out the needed metabolic activities.
      Usually prokaryotes are single-celled and don't have a true nucleus, but do have DNA. Prokaryote do not contain many organelles, but do contain ribosomes and a plasmid DNA. Ribosomes function in the sythesis of proteins. The plasmid DNA is used by the bacterium in the production of enzymes which defend them from viruses. We use plasmid DNA as a vector (carrier) in genetic engineering (lecture 20). Examples: Escherichia coli (E. coli): one strain of E. coli (the uncooked hamburger strain?) can kill you; other strains are not toxic.
      Prokaryotes reproduce by binary fission, (a process more primitive than asexual reproduction in eukaryotes). As a prokaryote grows, it duplicates its DNA, which then attaches to the cell membrane/ cell wall area; each duplicate separates with one half of the original cell, which divides into two cells, each containing a duplicate of the DNA. As each new cell grows, this process is repeated, repeatedly.
      Although we say prokaryotes have a single circular strand of DNA, it is still a rather extensive amount of material; (it's circular in that it doesn't have 'ends,' like a string: it's continuous).
      Other examples of prokaryotes: they are small: (remember the clusters on the point of a pencil). Our relationship to (need for) prokaryotes: cyanobacterium (blue-green algae) inocorporate nitrogen into living tissues. Without their doing so, we wouldn't be.
      We use prokaryotes: in technology (e.g. headphones); the bacteria such as that in Yellowstone's 'hot-pots,' produce enzymes that work at high temperatures (too high for our enzymes to work). These enzymes are used in genetic engineering. Prokaryotes eat (break down) just about anything: used in soaps, oil spills. Enzymes from another bacterium, by increasing the temperature at which water will freeze, help in making artificial snow. Yet another is used to kill the larvae that cause river blindness. When Ogden had a problem with the gypsy moth, it was solved by spraying a (biodegradable) bacterium.
    2. Eukaryotic cells ("true nucleus" - advanced cell type)   plant cell   animal cell
      Eukaryotes have a true nucleus. (Plant and animal cells are eukaryotic.) Eukaryotic cells are complex, highly organized, containing a variety of organelles. Some have a cell wall: plants (cell wall primarily of cellulose) and fungi (cell wall primarily of chitin). Animals: no cell wall. Outside boundary is a plasma membrane only.
      Eukaryotes reproduce asexually (mitosis lecture 11) and sexually (meiosis lecture 12). (e.g stramberry plant: 'runners' develop into individual plants. Strawberries also reproduce sexually: (flowers: male and female parts.) Asexual reproduction is very uncommon in animals, but not absent: aphids, for example, reproduce can reproduce asexually.
      Usually eukaryotes are multicellular, but can be single-celled. Eukaryotic organisms range in size from microns, such as the illions all over your body (e.g. mites), to relatively huge: whales, elephants, etc..
    3. Common features of prokaryotic and eukaryotic cells
      1. Cell coverings
        All cells have cell coverings.
        1. Cell wall
          All prokaryotes have cell walls. Plants and fungi (eukaryotes) have cell walls as well.
        2. Plasma membrane Plasma membrane
          All living cells, prokaryotic or eukaryotic, have plasma membrane. The plasma membrane is composed of a phospholipid bilayer. Each layer has a fatty end (always on the inside because it is non-polar) which is hydrophobic ("water-hating" fatty tails). The phosphorous end is charged (negative) and so it interacts with water ("water-loving" hydrophilic). The bilayer, which is self-assembling, contains proteins (various purposes: transport, etc.), cholesterol (prevent the phospholipids from packing too tightly).
      2. Cytoplasm
        The cytoplasm is everything in the cell but the cell wall, cell membrane, and nucleus.
      3. Organelles: Ribosomes
        Ribosomes are the common organelle in eukaryotes and prokaryotes. Because every cell must produce proteins at some point, ribosomes are required.
      4. Genetic information: DNA
        Both cell types contain DNA.
      5. Metabolic similarities
        Autotrophs and heterotrophs in both groups. Every cell must have an energy source (be able to process ATP).
        Review the summary table.