Genetic Engineering
Study Questions

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  1. What enzyme cuts the DNA at a specific nucleotide sequence?
  1. reverse transcriptase
  2. ligase enzyme
  3. restriction enzyme
  4. DNA cutase
  1. What enzyme splices the DNA back together?
  1. reverse transcriptase
  2. ligase enzyme
  3. restriction enzyme
  4. DNA stickase
  1. First, from organism A, we remove a segment of DNA that codes for the production of amylase (a salivary protein that digests starches). After inserting it into the DNA of a bacterial host, the host produces amylase. Accounting for these events (removal, insertion, production), which of the following is the correct sequence?
  1. restriction enzyme, protein synthesis, ligase enzyme
  2. ligase enzyme, restriction enzyme, replication
  3. ligase enzyme, protein synthesis, restriction enzyme
  4. restriction enzyme, ligase enzyme, protein synthesis
  1. Which of the following could be considered vectors? (check all that apply)
  1. plasmid DNA
  2. bacteria
  3. virus
  4. mammalian cells
  1. The term "sticky ends" refers to the fact that when a portion of DNA is removed by restriction enzymes, the ends left behind will adhere and eventually bond to other DNA.
TRUE
FALSE
  1. Taking an insulin gene from a human and combining it with plasmid DNA from a bacterium is an example of recombinant DNA formation.
TRUE
FALSE
  1. Recombinant DNA technology is the fastest way to obtain desired combinations of genes.
TRUE
FALSE
  1. Humans have been artificially selecting desirable traits in organisms for thousands of years.
TRUE
FALSE
MATCHING:
(use lower case; incorrect responses will be replaced with a "?".)

9. restriction enzyme a. DNA glue

10. ligase enzyme b. plamid DNA

11. vector c. sticky ends
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